The University of Defence Headquarters seats in the Moravian metropolis – Brno together with the Faculty of Economics and Management, Faculty of Military Technology, Institute of Operation and Tactical Studies and Institute of Strategic Studies. The school is situated in the vicinity of downtown close to significant colleges, research institutes and libraries.
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The Faculty of Military Medicine is stationed in Hradec Králové. Its campus neighbours Charles University faculties and its teaching hospital.
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The Institute of Protection Against the Mass Destruction Weapons is situated within the campus of the former Military University of the Ground Forces, Vyškov. The Institutes shares the campus with a departmental secondary school, training centre and other elements of the Czech Army.